Abstract:Slag, iron carbon, calcium bentonite, ordinary bentonite, biological ceramsite, volcanic rock, zeolite and gravel were selected as potential substrates in a constructed wetland. Firstly, adsorption isotherm test of eight single substrates was carried out. Then, the substrate combinations were optimized according to adsorption isotherm tests and the removal effect of pollutants were further investigated and analyzed. The results showed that when the initial TN and TP concentration of high salinity and refractory organic wastewater was about 20mg/L and 5mg/L, the removal rates of TP and TN by single slag reached 29.7% and 22.6%, respectively; the removal rates of TP and TN by slag and iron carbon combined substrate was 31.8% and 23.2%, showing the effect of "1+1>2". However, the removal effect of the substrate on ammonia nitrogen was poor. Therefore, it was necessary to cultivate and domesticate functional microorganisms to achieve ammonia nitrogen removal through biological action in the subsequent dynamic test of the constructed wetland system.