The rigid-frame bridge is a mature and widely used bridge type in bridge design. But generally, the long-span rigid-frame bridge is mainly vehicular bridge, and the comfort level under the pedestrian conditions is rarely checked. At present, the steel structure or composite structure bridges are mostly used for the long-span pedestrian bridges in urban construction. But in some special cases, the cast-in-situ structure has its irreplaceable advantages. Taking a single-span 50 m rigid-frame bridge as an example, the checking calculation method of the comfort level for single-span long-span cast-in-situ rigid-frame pedestrian bridge is introduced, which provides the experience and reference for similar projects.